Cell Host & Microbe
Volume 25, Issue 5, 8 May 2019, Pages 681-694.e8
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Article
Mucispirillum schaedleri Antagonizes Salmonella Virulence to Protect Mice against Colitis

https://doi.org/10.1016/j.chom.2019.03.004Get rights and content
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Highlights

  • Mucispirillum schaedleri confers protection against Salmonella colitis in mice

  • Salmonella and M. schaedleri compete for anaerobic respiration substrates in the gut

  • M. schaedleri restricts Salmonella infection and inhibits virulence factor expression

  • Mucispirillum spp. are enriched in human gut mucosal samples

Summary

The microbiota and the gastrointestinal mucus layer play a pivotal role in protection against non-typhoidal Salmonella enterica serovar Typhimurium (S. Tm) colitis. Here, we analyzed the course of Salmonella colitis in mice lacking a functional mucus layer in the gut. Unexpectedly, in contrast to mucus-proficient littermates, genetically deficient mice were protected against Salmonella-induced gut inflammation in the streptomycin colitis model. This correlated with microbiota alterations and enrichment of the bacterial phylum Deferribacteres. Using gnotobiotic mice associated with defined bacterial consortia, we causally linked Mucispirillum schaedleri, currently the sole known representative of Deferribacteres present in the mammalian microbiota, to host protection against S. Tm colitis. Inhibition by M. schaedleri involves interference with S. Tm invasion gene expression, partly by competing for anaerobic electron acceptors. In conclusion, this study establishes M. schaedleri, a core member of the murine gut microbiota, as a key antagonist of S. Tm virulence in the gut.

Keywords

colonization resistance
Agr2
anterior gradient protein 2 homolog
Oligo-MM
OMM
altered Schaedler flora
ASF
microbiome
Salmonella pathogenicity island
SPI-1
T3SS
nitrate respiration

Cited by (0)

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These authors contributed equally

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Present address: UMR 1071 Inserm, USC, INRA 2018, Microbes, Intestine, Inflammation and Host Susceptibility, University of Clermont Auvergne, Clermont-Ferrand, France

13

Present address: Institute of Microbiology, Department of Biology, ETH Zürich, Zurich, Switzerland

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